The treatment of the V79 cells with the reactive oxidant radical generator, hypoxanthine/xanthine oxidase (X/XO) and hydrogen peroxide, resulted in a dose and time dependent increase in the intracellular salicylic acid hydroxylation products: 2,3-dihydroxybenzoic acid (2,3-DHBA) and 2,5-dihydroxybenzoic acid (2,5-DHBA). The formation of hydroxyl free radicals was also detected in cultures that were treated with hydrogen peroxide. However, treatment of salicylic acid loaded V79 cells with NITE WHITE with ACP gel and its placebo at doses from 0 to 100 mg/mL resulted in no increases in 2,3-DHBA and 2,5-DHBA.


CONCLUSION


It is concluded that NITE WHITE with ACP Tooth Whitening Gel does not induce the formation of intracellular hydroxyl free radicals in cultured V79 cells.